An experimental study of the effect of focusing the optical system of a microscope on the textural characteristics of the images of the bone marrow cells nuclei

This work relates to the direction of automation of medical diagnostics using computer microscopy. The effect of focusing a microscope on the textural characteristics of chromatin images of the nuclei of bone marrow cells in the computer microscopy system when solving diagnostic problems in oncomorphology for the recognition of malignant tumors is investigated. These questions are of particular importance when solving the problem of analyzing images of low-contrast objects-chromatin of the nucleus of bone marrow cells in the diagnosis of dangerous oncological diseases of the blood system-acute leukemia. During the experiment, bone marrow preparations from patients with acute lymphoblastic leukemia were used as test samples. The preparations were provided by the laboratory of hematopoiesis immunology of the N.N. Blokhin National Medical Research Center of Oncology. The results of the experiment among the characteristics of images of the structure of the chromatin of the nuclei of bone marrow cells revealed the high sensitivity of the focusing optical system of the microscope texture characteristic «moment of inertia» of the red components R of RGB color model. Practical recommendations are given for developers of automated systems on the use of the texture analysis apparatus in the design of cancer diagnostics systems based on microscopic methods of studying samples of biological materials.

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