Modeling and experimental verification of the biocell separation processes by the magnitude of magnetic susceptibility

In this article, suggestions are given for modeling the processes of cell separation by the magnitude of magnetic susceptibility. On the basis of the proposed model, an operating separation unit was created, and its description was given. To improve the efficiency of the operating separation unit, in comparison with existing equivalents, design solutions were introduced. It has been experimentally established that a lattice, consisting of rods of soft magnetic steel, which is a spatially periodic polygradient structure, can secrete cells of various magnetic susceptibility when the magnitude of the external magnetic field, applied to this structure, and the constant flow rate of the working medium, pumped through it, change. Additionally, we have also developed a technique for isolating cells with different magnetic susceptibility by means of the operating unit. The derived method makes it possible to acquire "spectra" of the magnetic susceptibility of cell samples. After the calibration of the unit, it is possible to predict the expected results of the cell separation analysis. The efficiency of the devised separator rises with an increase in the number of rows (rods) in the periodic structure. The accuracy of the findings depends on the pumping time. With weak fields, the shutter speed needs to be accelerated. Non-magnetic or weakly magnetic biological cells may remain in the swirl zones. This phenomenon negatively affects the quality of the samples obtained, especially with weak magnetic fields.

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